HTML5 is sure to be a big hit this year as far as web design trends go, and rightfully so. It is a revolutionary language that has totally changed how things were done before. It also ensures backward compatibility and some also have sub title support. It also, partially replaces the object element.
Among other great features, the new HTML5 specification allow native audio streaming. So here in this article I have collected very useful free and premium HTML5 audio players. These are 100% HTML5 based and do not required Flash or any other ActiveX controls to stream and play music on your site.
1. jPlayer
jPlayer is the completely free and open source (GPL/MIT) media library written in JavaScript.

2. HTML5 Audio Player with Playlist
Restyle the whole player with basic CSS into anything you want! (remove any feature from the player you dont need) 4 demo examples included in the download package!

3. audio.js
audio.js is a drop-in javascript library that allows HTML5’s <audio> tag to be used anywhere.

4. MooTools HTML5 Audio Player
This audio player supports all popular browsers like safari, Firefox, Chrome. Get the free source code and use this player.

5. JME
jme is an HTML5 audio / video development kit with Flash Fallback, which focuses on flexibility, intuitive DOM-API and semantic code.

6. Simple HTML5 Audio Player V1
The HTML5 Audio Player V1 is a simple and elegant looking HTML5 /Flash audio playing solution with some thoughtful features.

7. Universal HTML5 Audio Player
Unfortunately overlays do not work on browsers which do not support simultaneous <audio> element playback, such as current versions of iOS.

8. Accessible Audio Player (AAP)
AAP is available in a standalone version or (with an additional P) as a WordPress plugin called Accessible Audio Player Plugin (AAPP).

9. MusicBox – HTML5 Music Player
Due to html5 restrictions, this player requires both mp3 and ogg files for each song. There are many FREE applications that convert file types.
